Animal Cells Protein Lysis Buffer, 1x Strength Solution: The Animal Cells Protein Lysis Buffer extracts cytoplasmic and nuclear protein from cultured mammalian cells. The buffer utilizes mild non-ionic detergent with our proprietary combination of various salts and reagents to enhance protein extraction and stability. The buffer is compatible with many applications, including reporter assays (e.g., luciferase, β-galactosidase, acetyltransferase), protein assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blot, ELISA, RIA) and protein purification. This dialyzable reagent enables simple, rapid, mild and efficient lysis from a wide variety of Mammalian cell.