Lyisis-EZ J Denaturing Cell Lysis Buffer, 1x Strength Solution: The denaturing Cell Lysis Buffer is suitable for cell lysis of the following proteins: (1) The insoluble antigen, such as cytoskeleton and membrane protein, hydrophobic protein. (2) The proteins, epitopes of which are hidden in secondary even higher structure and some proteins were subjected to aggregate and precipitate. (3) The proteins translated in vitro, treated protein can be used for downstream experiments such as SDS-PAGE, 1D & 2D electrophoresis, 10ml of the Buffer is sufficient for 10x107 cells or 10x100mg tissue sample.